GFP Expression in Bacteria

Research on specific gene expression in bacteria has been greatly facilitated by the use of reporter genes. Green fluorescent protein (GFP) and its variants are powerful reporter proteins. There are several advantages of using green fluorescent protein (GFP) in microbiological analyses. One advantage of green fluorescent protein (GFP) is that its expression does not require any substrate addition. The GFP gene can be expressed and visualized in different types of organisms, such as plant leaves, roots, seeds, zebra fish, mice and a wide variety of bacteria. Green fluorescent protein (GFP) is an excellent tool for non-destructive studies of bacterial communities or other systems where live cells are studied at the single-cell level.

How to Visualize GFP Expression in Bacteria?

GFP-expressing bacteria can be easily identified and differentiated by green fluorescence when excited with Fluorescence Flashlight GFPfinder-2101RB.

Fluorescence Flashlight GFPfinder-2101RB provides uniform, intense blue light of 450 nm, which is the optimal wavelength to excite green fluorescent protein (GFP) in bacteria. The emitted green fluorescence can be viewed through matching barrier filter LUV-30A, and GFP-positive bacteria can be easily identified and differentiated. Using our supplied camera filter, researchers can take high-quality photos of GFP fluorescence for publication.

Photo below: GFP expression in bacteria visualized using Fluorescence Flashlight GFPfinder-2101RB (photo provided by Southwest University)

Visualize Other Fluorescent Proteins?

If you are working on other fluorescent proteins (e.g., mChery, DsRed, YFP, DAPI), Fluorescence Flashlight GFPfinder-2101 can also do the job! Fluorescence Flashlight GFPfinder-2101 offers 5 different wavelenths: 365 nm, 400 nm, 450 nm, 485 nm, and 520 nm. Simply choose the wavelength based on your fluorescent protein.

Photo below: Fluorescence Flashlight GFPfinder-2101 designed for detecting fluorescent proteins in bacteria, plants, and animals.