Which GFPfinder excitation and emission combination should I use for my fluorophore (GFP, FITC, RFP…)?
The XEPU GFPfinder Flashlights offer five different fluorescence excitation wavelengths, which can be used to excite a wide range of fluorophores. This page provides guidance on selecting the right wavelength set for your application.
The table below lists the GFPfinder wavelength sets, including the most intense part of the excitation range and the cutoffs of the paired emission filters.
Our recommendations are based on either working knowledge with that fluorophore – our own or feedback from customers – or on published spectral data.
Designation | Excitation | Emission |
UV– Ultraviolet | 360 – 380nm | 415nm longpass |
VI– Violet | 400 – 415nm | 450nm longpass |
RB– Royal Blue | 440 – 460nm | 500nm longpass |
RB– Royal Blue | 440 – 460nm | 500 – 560nm bandpass |
CY– Cyan | 490 – 515nm | 550nm longpass |
GR– Green | 510 – 540nm | 600nm longpass |