Which Excitation Wavelength Should I Use for My Fluorophore?

Published: 2024-01-11
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Which GFPfinder excitation and emission combination should I use for my fluorophore (GFP, FITC, RFP…)?

The XEPU GFPfinder Flashlights offer five different fluorescence excitation wavelengths, which can be used to excite a wide range of fluorophores. This page provides guidance on selecting the right wavelength set for your application.

The table below lists the GFPfinder wavelength sets, including the most intense part of the excitation range and the cutoffs of the paired emission filters.

Our recommendations are based on either working knowledge with that fluorophore – our own or feedback from customers – or on published spectral data.

DesignationExcitationEmission
UV– Ultraviolet360 – 380nm415nm longpass
VI– Violet400 – 415nm450nm longpass
RB– Royal Blue440 – 460nm500nm longpass
RB– Royal Blue440 – 460nm500 – 560nm bandpass
CY– Cyan490 – 515nm550nm longpass
GR– Green510 – 540nm600nm longpass


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