Enhanced Green Fluorescent Protein (EGFP)

Enhanced green fluorescent protein (EGFP) is a widely used variant of GFP. Compared with wild-type GFP, EGFP is brighter and matures rapidly at 37°C. EGFP is a powerful tool for the visualization of tagged proteins and transfected cells in molecular and biological research.

To detect EGFP fluorescence, the first step is to learn the excitation and emission wavelength of EGFP. Based on the spectrum of EGFP fluorescent protein, choose a fluorescence detector that has an appropriate excitation wavelength for EGFP. As shown in the following figure, EGFP can be excited by the 488 nm laser line and is optimally detected at 507 nm.

Figure below: excitation and emission wavelength of EGFP fluorescent protein

Detect EGFP Fluorescent Protein Using GFPfinder-2101RB

GFPfinder-2101RB Fluorescence Flashlight is a powerful tool to detect GFP and EGFP fluorescent proteins in plants, leaves, seeds, roots, cells, and animals. Using GFPfinder-2101RB, you can detect GFP and EGFP fluorescence in seconds! 

Features of GFPfinder-2101RB Fluorescence Flashlight

● Wide applications: GFPfinder-2101RB Fluorescence Flashlight can be used to detect GFP and EGFP fluorescent proteins in plants, leaves, seeds, roots, cells, and animals (e.g. mice, zebra fish).

● Intense excitation light: Equipped with 12 watt high-power LEDs, GFPfinder-2101RB Fluorescence Flashlight can detect weak fluorescence in the dark!

● Portability: Powered by a rechargeable battery, you can use GFPfinder-2101RB Fluorescence Flashlight in the field and detect EGFP fluroescence in living plants.

● Efficiency: No pre-heating is required. Turn on the flashlight, and detect EGFP fluorescence in seconds with the naked eye!

Figure below: EGFP fluorescence in leaves detected by GFPfinder-2101RB Fluorescence Flashlight

Figure below: GFPfinder-2101RB designed for the detection of GFP and EGFP fluorescence